f nucleatum strains fnp atcc 10953  (ATCC)

Journal: bioRxiv

Article Title: RadD from Fusobacterium nucleatum Engages NKp46 to Promote Antitumor Cytotoxicity

doi: 10.1101/2025.07.26.666929

Figure Lengend Snippet: NKp46 interaction with RadD expressed by Fusobacterium nucleatum triggers NK cell cytotoxicity. This activation enhances tumor cell killing in vitro and in vivo . Conversely, the absence of RadD or the blocking of NKp46 impairs NK cell activity, leading to tumor progression. Created by BioRender.

Article Snippet: To identify the F. nucleatum ligand of NKp46, we assessed the binding of NKp46 Ig, its D1 domain (D1 Ig), its mouse orthologue Ncr-1 Ig and CD16 Ig to FITC-labeled F. nucleatum strains ATCC 10953 and ATCC 23726 , which represent the subspecies polymorphum and nucleatum , respectively ( ).

Techniques: Activation Assay, In Vitro, In Vivo, Blocking Assay, Activity Assay

Journal: iScience

Article Title: Fusobacterium nucleatum subsp. nucleatum RadD binds Siglec-7 and inhibits NK cell-mediated cancer cell killing

doi: 10.1016/j.isci.2024.110157

Figure Lengend Snippet: Siglec-7 binding to F. nucleatum strains (A) FITC-labeled F. nucleatum subsp. nucleatum ATCC 23726 ( Fnn 23726) was incubated with 2 μg of Siglec-7-Ig, NTB-A-Ig, 2B4-Ig, or CD16-Ig and binding was revealed with fluorescently labeled secondary antibodies. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of three is shown. (B) Two strains of F. nucleatum subsp. nucleatum ( Fnn 23726 and Fnn 25586) and two strains of F. nucleatum subsp. polymorphum ( Fnp 10953 and Fnp 12230) as well as (C) a clinical strain of F. nucleatum ( Fnn CTI-7) were incubated with Siglec-7-Ig. Filled gray histograms represent staining with secondary antibody only. One representative experiment out of two is shown.

Article Snippet: While Fnn 23726 and Fnn 25586 both bound to Siglec-7-Ig, F. nucleatum subsp. polymorphum strains ATCC 10953 and 12230 (subsequently abbreviated as Fnp 10953 and Fnp 12230) showed little to no staining.

Techniques: Binding Assay, Labeling, Incubation, Staining

Journal: mBio

Article Title: Comparative Analysis of Colon Cancer-Derived Fusobacterium nucleatum Subspecies: Inflammation and Colon Tumorigenesis in Murine Models

doi: 10.1128/mbio.02991-21

Figure Lengend Snippet: F. nucleatum strains used in this study

Article Snippet: Previous genomic analysis of the F. nucleatum subsp. polymorphum strain ATCC 10953 revealed that 25% of the protein-coding genes were unique to that strain, with evidence of horizontal gene transfer with Firmicutes , particularly Clostridia ( ).

Techniques:

Journal: mBio

Article Title: Comparative Analysis of Colon Cancer-Derived Fusobacterium nucleatum Subspecies: Inflammation and Colon Tumorigenesis in Murine Models

doi: 10.1128/mbio.02991-21

Figure Lengend Snippet: Differential colonization of F. nucleatum subspecies in SPF and GF wild-type (WT) mice. (A) SPF mice were orally gavaged with the designated F. nucleatum subspecies at days 0, 7, 14, and 21 (arrows), and fecal pellets were collected at the indicated time points on the x axis. F. nucleatum colonization is plotted as F. nucleatum copies/ng fecal DNA per group over time (mean ± SD), with a detection limit (LOD) of 100 F. nucleatum copies. n = 3 control mice and 10 experimental mice per strain. (B) GF WT mice were orally gavaged with F. nucleatum once and then assessed for colonization after 14 days. Each dot indicates the F. nucleatum copies/ng DNA of an individual mouse. Bars indicate the median with interquartile range. ( Fna denotes F. nucleatum subsp. animalis , Fnn denotes F. nucleatum subsp. nucleatum , Fnv denotes F. nucleatum subsp. vincentii , Fnp_c denotes clumpy F. nucleatum subsp. polymorphum , and Fnp _ nc denotes nonclumpy F. nucleatum subsp. polymorphum ). n = 5 to 17 mice/group. One GF control mouse calculated as having 125 F. nucleatum copies/ng DNA was removed from analysis.

Article Snippet: Previous genomic analysis of the F. nucleatum subsp. polymorphum strain ATCC 10953 revealed that 25% of the protein-coding genes were unique to that strain, with evidence of horizontal gene transfer with Firmicutes , particularly Clostridia ( ).

Techniques: Control

Journal: mBio

Article Title: Comparative Analysis of Colon Cancer-Derived Fusobacterium nucleatum Subspecies: Inflammation and Colon Tumorigenesis in Murine Models

doi: 10.1128/mbio.02991-21

Figure Lengend Snippet: Changes in distal colon inflammatory gene expression. GF WT mice were inoculated with the indicated F. nucleatum strains then harvested after 14 days. RNA was extracted from distal colon tissue, and relative quantification of gene expression was performed with qRT-PCR using TaqMan gene expression Assays for each target gene, normalized to murine GAPDH. Data are plotted as the relative expression (threshold cycle [2 −ΔΔ CT ]) per mouse. Bars indicate the median with interquartile range. n = 5 to 17 mice/group. ( Fna denotes F. nucleatum subsp. animalis , Fnn denotes F. nucleatum subsp. nucleatum , Fnv denotes F. nucleatum subsp. vincentii , Fnp_c denotes clumpy F. nucleatum subsp. polymorphum , and Fnp _ nc denotes nonclumpy F. nucleatum subsp. polymorphum ).

Article Snippet: Previous genomic analysis of the F. nucleatum subsp. polymorphum strain ATCC 10953 revealed that 25% of the protein-coding genes were unique to that strain, with evidence of horizontal gene transfer with Firmicutes , particularly Clostridia ( ).

Techniques: Gene Expression, Quantitative Proteomics, Quantitative RT-PCR, Expressing

Journal: mBio

Article Title: Comparative Analysis of Colon Cancer-Derived Fusobacterium nucleatum Subspecies: Inflammation and Colon Tumorigenesis in Murine Models

doi: 10.1128/mbio.02991-21

Figure Lengend Snippet: Colonization and tumorigenesis in F. nucleatum -treated GF Apc Min/+ mice. GF Apc Min/+ mice were orally gavaged once per week for 4 weeks (arrows), and fecal pellets were collected at the indicated time points on the x axis. (A) Colonization is plotted as F. nucleatum copies/ng fecal DNA per group over time (mean ± SD) with a detection limit (LOD) of 100 F. nucleatum copies. (B) Data are displayed as number of tumors/colon per mouse. Bars indicate the median with interquartile range. The number of mice with tumors out of total mice inoculated with each strain are displayed above the graph. n = 6 to 12 mice/group. ( Fna denotes F. nucleatum subsp. animalis , Fnn denotes F. nucleatum subsp. nucleatum , Fnv denotes F. nucleatum subsp. vincentii , Fnp_c denotes clumpy F. nucleatum subsp. polymorphum , and Fnp _ nc denotes nonclumpy F. nucleatum subsp. polymorphum ).

Article Snippet: Previous genomic analysis of the F. nucleatum subsp. polymorphum strain ATCC 10953 revealed that 25% of the protein-coding genes were unique to that strain, with evidence of horizontal gene transfer with Firmicutes , particularly Clostridia ( ).

Techniques:

Journal: mBio

Article Title: Comparative Analysis of Colon Cancer-Derived Fusobacterium nucleatum Subspecies: Inflammation and Colon Tumorigenesis in Murine Models

doi: 10.1128/mbio.02991-21

Figure Lengend Snippet: Changes in distal colon inflammatory gene expression in GF Apc Min/+ mice. GF Apc Min/+ mice were orally gavaged once per week for 4 weeks with the indicated strains, and distal colons were harvested at 11 weeks. Mice were only included in the analysis if they remained stably colonized for the duration of the experiment. (Therefore, strain CRC- F. nucleatum subsp. nucleatum is excluded from this figure.) Data are plotted as the relative expression (2 −ΔΔ CT ) per mouse. Bars indicate the median with interquartile range. All P values of <0.05 were considered significant. n = 3 to 6 mice/group. ( Fna denotes F. nucleatum subsp. animalis , Fnn denotes F. nucleatum subsp. nucleatum , Fnv denotes F. nucleatum subsp. vincentii , Fnp_c denotes clumpy F. nucleatum subsp. polymorphum , and Fnp _ nc denotes nonclumpy F. nucleatum subsp. polymorphum ).

Article Snippet: Previous genomic analysis of the F. nucleatum subsp. polymorphum strain ATCC 10953 revealed that 25% of the protein-coding genes were unique to that strain, with evidence of horizontal gene transfer with Firmicutes , particularly Clostridia ( ).

Techniques: Gene Expression, Stable Transfection, Expressing

Journal: mBio

Article Title: Comparative Analysis of Colon Cancer-Derived Fusobacterium nucleatum Subspecies: Inflammation and Colon Tumorigenesis in Murine Models

doi: 10.1128/mbio.02991-21

Figure Lengend Snippet: Secretion of CXCL1 (A) and IL-8 (B) from F. nucleatum -treated human HCT116 cells. HCT116 cells were incubated with F. nucleatum strains at an MOI of 50:1 for 4 h, and supernatants were analyzed by ELISA, performed in triplicate. Data are presented as mean ± SD. All strains depicted herein are CRC-derived isolates. For non-CRC isolates, see Fig. S6 in the supplemental material. ( Fna denotes F. nucleatum subsp. animalis , Fnn denotes F. nucleatum subsp. nucleatum , Fnv denotes F. nucleatum subsp. vincentii , Fnp_c denotes clumpy F. nucleatum subsp. polymorphum , and Fnp _ nc denotes nonclumpy F. nucleatum subsp. polymorphum ).

Article Snippet: Previous genomic analysis of the F. nucleatum subsp. polymorphum strain ATCC 10953 revealed that 25% of the protein-coding genes were unique to that strain, with evidence of horizontal gene transfer with Firmicutes , particularly Clostridia ( ).

Techniques: Incubation, Enzyme-linked Immunosorbent Assay, Derivative Assay

Journal: mBio

Article Title: Comparative Analysis of Colon Cancer-Derived Fusobacterium nucleatum Subspecies: Inflammation and Colon Tumorigenesis in Murine Models

doi: 10.1128/mbio.02991-21

Figure Lengend Snippet: (A) Phylogenetic tree of 18 previously published whole-genome sequences of F. nucleatum strains aligned with 5 genomes newly sequenced for this study (in bold and marked with an asterisk). (B) Venn diagram depicting genomic comparison of the five sequenced CRC-derived F. nucleatum strains. Each strain is represented by a colored oval. The numbers represent the predicted protein coding genes unique to or shared by each strain, based on an 80% identity requirement. ( Fna denotes F. nucleatum subsp. animalis , Fnn denotes F. nucleatum subsp. nucleatum , Fnv denotes F. nucleatum subsp. vincentii , Fnp_c denotes clumpy F. nucleatum subsp. polymorphum , and Fnp _ nc denotes nonclumpy F. nucleatum subsp. polymorphum ).

Article Snippet: Previous genomic analysis of the F. nucleatum subsp. polymorphum strain ATCC 10953 revealed that 25% of the protein-coding genes were unique to that strain, with evidence of horizontal gene transfer with Firmicutes , particularly Clostridia ( ).

Techniques: Comparison, Derivative Assay